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Research Update

5-Methyl C and 5-Hydroxymethyl C Reduce Contamination in mtDNA NGS

Next generation sequencing (NGS) has transformed the landscape of genome sequencing making it quicker, cheaper and more accurate. Several NGS platforms exist and they often use universal primers (primers that are complementary to common sequences in a specific set of DNA molecules or plasmids). While universal primers offer a clear advantage, they can lead to contamination and amplification of unwanted products. In a recent paper, Expanded Genetic Codes in Next Generation Sequencing Enable Decontamination and Mitochondrial Enrichment, McKernan and colleagues present a method that ensures serial amplification steps can be performed without contamination, even without physical isolation of lab equipment.

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TriLink Awarded Patents for Novel Ligation Technology

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5-me-dCTP & 5-hme-dCTP, new tools in mtDNA sequencing, are also key epigenetics research reagents.

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Latest Blog Post

Smartphone Science: Nifty Accessories for
Bio-Medical Applications

  • DIY Cholesterol and Vitamin D Monitoring Using Smartphones
  • Single-molecule Microscopy on Smartphones…Coming Soon?
  • Pee-powered Smartphones Developed

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Right: Prof. David Erickson using his iPhone interfaced with a blood test strip. Photo credit: Jason Koski/University Photography; taken from via Bing Images.

Improving Sequencing Reads of GC-rich DNA Targets
GC-rich DNA targets can be difficult to sequence due to the high degree of intrastrand secondary structure formation. The quality of sequencing results for GC-rich regions can be compromised by loss of signal, band compressions and weak signal base calls. PCR amplification of these problematic targets with the CleanAmp™ 7-deaza-dGTP Mix prior to Sanger dideoxy sequencing can significantly improve the read quality along the entire sequence.   GC Sequencing
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Cyanine 5 FLuc mRNA
When labeled with cyanine 5, FLuc mRNA can be directly visualized. Cyanine 5 FLuc mRNA is an ideal molecule to determine mRNA delivery and localization independent of translation.

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Previous Blog Post

Artificially Expanding DNA’s Genetic Code in Designer Microbes
  • DNA isn’t What it Used To Be!
  • Scripps Team Led by Floyd Romesberg Demonstrates Six-Base DNA Replication in Living Bacteria
  • Are We On Our Way to Semi-synthetic Life-forms? With Vast Potential—But at What Risks?

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