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Research Update

The C-terminal domain of p53 influences target DNA binding and complex stability by altering p53 structure

The transcription factor p53 is a structurally complex protein at the hub of many signaling pathways that regulate the cell cycle and maintain the integrity of the genome. The cell fate decisions of p53 range from blocking proliferation to inducing cell-cycle arrest to differentiation or apoptosis. The role of p53 in cell fate control is underscored by the fact that an estimated 50% of malignancies harbor a mutation within the p53 locus. Because of this ability to control life and death, p53 has been dubbed ‘the guardian of the genome’ and has been studied extensively; however its complex structure has been limiting.

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New Poster at RNA Society
Learn more about how novel nucleic acid modifications affect mRNA translation.
New Product
Improve fast cycling and multiplex PCR with CleanAmp™ Turbo Primers.
Come Out and See Us
RNA Society May 26-31.
MicroRNAs & Noncoding RNAs in Cancer June 7-12.

Resolve RNA-Protein Interactions to Individual Nucleotides with the 3' iCLIP Primer

UV crosslinking and immunoprecipitation (CLIP) was developed to identify RNA-protein interactions. While several versions of the CLIP method now exist, individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) allows identification of the site of RNA-protein interaction at high resolution.

With the iCLIP method, the RNA is reverse transcribed after the UV crosslinking and immuprecipitation steps.  This creates cDNAs that are truncated at the crosslink site.  While this allows for the identification of the site of interaction there is often poor amplification of the truncated cDNA. However, now you can circumvent this amplification problem using TriLink’s 3' iCLIP Primer.

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iCLIP utilizes UV crosslinking to resolve protein-RNA interactions down to a single nucleotide. Figure adapted from Huppertz I et. al. Methods. 2014 Feb;65(3):274-87.

Ask An Expert

I just used your CleanTag™ Ligation Kit for Small RNA. Should I expect to see essentially no adaptor dimer formation?

Dear Steve,

You are correct in that the CleanTag™ chemistry can fully suppress adapter dimer formation. If you are using very low input levels (less than 10 ng), you may start to see some adapter dimer but it should be minimal compared to amount of tagged library.

Read the full question and answer here.

   

Zone In with Zon Blog Post

Gene-Expression Biomarkers Can Detect Depression

  • First-ever Lab Test for Depression Found Using RT-PCR
  • FDA Approval as Diagnostic Possible by Early 2016
  • Huge Potential Market as 1-in-10 US Adults Suffer from Depression
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Magnetic Resonance Imaging (MRI) images of hippocampus (yellow) and amygdala (blue), which are symmetrically located in both lobes of the brain (upper right)

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