|Resolve RNA-Protein Interactions to Individual Nucleotides with the 3' iCLIP Primer
UV crosslinking and immunoprecipitation (CLIP) was developed to identify RNA-protein interactions. While several versions of the CLIP method now exist, individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) allows identification of the site of RNA-protein interaction at high resolution.
With the iCLIP method, the RNA is reverse transcribed after the UV crosslinking and immuprecipitation steps. This creates cDNAs that are truncated at the crosslink site. While this allows for the identification of the site of interaction there is often poor amplification of the truncated cDNA. However, now you can circumvent this amplification problem using TriLink’s 3' iCLIP Primer.
iCLIP utilizes UV crosslinking to resolve protein-RNA interactions down to a single nucleotide. Figure adapted from Huppertz I et. al. Methods. 2014 Feb;65(3):274-87.