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Research Update

Using Small RNA Sequencing to Improve Diagnosis and Treatment of Coronary Artery Disease

Using next-generation sequencing (NGS) and CleanTag™ technology, French researchers discovered that a subclass of circulating small non-coding RNAs (sRNAs) is a biomarker for apoptotic lesional macrophages commonly associated with coronary artery disease (CAD). Currently, CAD lacks specific diagnostic criteria and is the leading cause of death worldwide. This discovery may enable a specific diagnostic avenue not previously available and aid in improving treatment through risk stratification.

"We are very close to predict, with just a blood withdrawal, if and when a heart attack may occur." - Dr. Emanuela Repetto.

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Reduce Adapter Dimer with our New CleanTag™ Ligation Kit for Small RNA Library Preparation

TriLink’s new CleanTag™ technology blocks adapter dimer formation during the ligation steps of small RNA library preparation.

Our New CleanTag™ Ligation Kit for Small RNA Library Prep:

  • Increases mappable sequencing reads even with ultra low RNA inputs
  • Tags efficiently in samples with low miRNA abundance
  • Is suited for diagnostic samples with limited RNA
  • Eliminates the need for gel purification
  • Enables full automation
  • Is compatible with Illumina and Ion Torrent platforms

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Ask an Expert

My Bioanalzyer® peaks seem to be shifted higher during analysis. Is this normal?

Buffer components may cause higher base pair shift in crude Bioanalyzer® traces as show below. miRNA peak should be at ~150 bp for crude samples and at ~140 bp when...

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Zone In With Zon Blog Post

DNA Barcodes to Follow Food and Foil Fraud

Nifty work by researchers who have applied qPCR to detect DNA barcode “tags” to follow foodstuff from your fork back to the farm.

Zone In on November 17th for my new in depth blog post "Small RNA is Big Science."

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