Modified oligos from TriLink used to synthesize polymer-tagged nucleotides for new nanopore sequencing method
In an eNews article a while back, the 5’hexynyl group was highlighted as one possible oligo modification offered by TriLink to enable click chemistry, a very versatile conjugation method. There are many applications for click chemistry, and recently TriLink 5’ hexynyl-modified oligonucleotides were used for a new nanopore sequencing method in which click chemistry is utilized to synthesize polymer-tagged nucleotides.
Nanopore sequencing is normally carried out by threading a strand of DNA through a protein-based nanopore and converting resultant ionic current fluctuations into nucleotide base sequence. A radically different strategy using 5’ hexynyl-modified oligonucleotides was recently reported in...