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What are your recommended conditions for running a gel for mRNA or long RNA transcripts?
We run a 1% agarose gel. Depending on the size of the gel/number of wells, we load 250 ng to 1 ug of mRNA. We dilute mRNA to 0.2 ug/uL, then add equal volume of NorthernMax Gly sample loading dye.
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Top 10 Innovations 2016
As in the past, this first blog of the year comments on the Top 10 Innovations in 2016 that were selected by a panel of judges and published last month in The Scientist. So, with an imaginary loud flourish of trumpets, read on to learn about the 10 winners, starting with 1st place....
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