Twitter Facebook Linked In Zone In Google Plus 
July 2018 Research Update

Closer to a Cure? Elucidating the Structure of the HIV Reverse Transcriptase Initiation Complex

Last month, in a study published in Nature, a team of researchers from Stanford University unraveled the structural basis of the HIV reverse transcriptase initiation complex (RTIC), the discovery of which may have brought us closer to a cure for HIV.

Although the structure of each HIV component has previously been elucidated, the structural basis of the initiation of transcription has remained elusive despite lasting efforts to uncover it.

Understanding this process provides new opportunities for drug targeting, which could be essential for maintaining control of the rapidly mutating virus.

"A rich body of structural data on reverse transcriptase (RT), a heterodimer of p51 and p66 sub-units, has shown how its polymerase and RNase H domains interact with DNA-DNA and DNA-RNA duplexes in the absence and presence of antiviral drugs. Lacking, however, are structures that reflect initiation, showing how RT binds to a large bimolecular viral RNA(vRNA)-tRNALys complex," the authors explain.

Using a combination of cryo-electron microscopy, biochemical, and biophysical experiments, this group of researchers built an RTIC model. In this model, the RT is in an inactive conformation with open fingers and thumb, while the primer-template complex is turned away from...

Read More
 
Oligonucleotides for IVD & Molecular Dx
TriLink diagnostic oligo products include robust DNA and RNA modification options. Our expertise and extensive selection of modifications and conjugates ensure that we can meet your molecular diagnostic oligonucleotide synthesis requirements.
Bioconjugation Services
Using our Chromalink® technology, our team of bioconjugation experts can perform custom conjugations to any biomolecule, including antibodies, oligos, proteins, peptides, aptamers, and even solid surfaces and magnetic beads.
OligoBuilder
Get instant pricing & ordering for modified and standard oligos from 0.2 µmole to 15 µmole synthesis scales.
The New Standard for
Superior mRNA Translation

Successful 5' capping is a critical characteristic for creating viable mRNA constructs that remain biologically active and avoid self/non-self intracellular immune stimulation. TriLink scientists recognized the limitations of current capping methodologies and have developed a new high efficiency, simplified co-transcriptional 5' capping reagent.

CleanCap™ - A Novel New mRNA Capping Technology:

  • 90 - 99% capping efficiency

  • Natural Cap 1 mRNA to reduce cellular innate immune response

  • Simplified "one-pot" transcription and 5' capping process

  • Lowest cost per milligram of biologically
    active Cap 1 mRNA

CleanCap mRNA is in stock; available for common applications & experiments in standard constructs or as a custom mRNA synthesis to your specifications. TriLink CleanCap reagent is also available for use in your lab's mRNA transcription procedures.

 
CRISPR
 
Ask An Expert

Are the Requirements for Using CleanCap mRNA Different Than ARCA mRNA?

The requirements for using CleanCap mRNA are no different than using ARCA mRNA. You can use your standard electroporation or transfection conditions. There are several advantages with using a CleanCap mRNA. The translational efficiency far exceeds that of a standard ARCA capped mRNA...

Read More
Zone In With The Zon Blog Post

ATP Synthase and ATPase: In Memory of Paul D. Boyer and Jens C. Skou

• ATP Is the "Molecular Unit of Currency" of Intracellular Energy Transfer

• 1997 Nobelists Boyer and Skou Elucidated Fundamental ATP Biochemistry


View Blog Post


 

 
 
© 2018 TriLink BioTechnologies | All Rights Reserved