Contact Us

Inorganic Pyrophosphatase (iPPase) - (E-0051)

In stock
default
E-0051
Product SKU Unit Size Price Qty
E-0051-003 30 µg
$60.00
E-0051-03 310 µg
$480.00
Description

E. coli inorganic pyrophosphatase (iPPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate. Using iPPase in an in vitro transcription reaction drives RNA synthesis forward by removing pyrophosphate which forms as a byproduct and precipitates with key transcription buffer cations.

The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert.

Product details
Catalog No E-0051
Buffer 20 mM Tris, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 8.0
Reaction Sizes 25, 250
Volume 60 μL, 660 μL
Concentration 0.5 mg/mL, approximately 0.1 U/μL
Host E. coli
Recommended Storage -20 to -25°C
Technical documents

Safety Data Sheet Look-up

E-0051 Product Insert

Products FAQs

ISO 13485 compliant.

Typically, we recommend 0.002U/mL.