mCAP - (N-7001)
m7G(5')ppp(5')G Cap Analog, N7-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine
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N-7001
Description
Eukaryotic and viral messenger RNA (mRNA) has a monomethylated cap structure, m7G(5')ppp(5')G or mCAP, at the 5’-terminus. The cap analog plays an important role in cellular processes, most notably the initiation of mRNA translation. The cap interacts with different binding proteins, initiation factors and elements of ribosomes.
mCAP was the first cap analog to be introduced to the molecular biology toolbox. Half of the time, mCAP inserts in the correct orientation to enhance translation. The other 50% of molecules are not substrates for efficient translation, reducing the specific activity of the transcript. More recently, Anti Reverse Cap Analog (ARCA) was introduced. ARCA can only insert in the proper orientation, resulting in mRNAs that are translated twice as efficiently as those initiated with mCAP.
mCAP is incorporated in a transcription by including a mixture of the cap analog and GTP (usually at a 4:1 ratio). Approximately 80% of synthesized mRNA will possess a 5’ cap, while the remaining 20% will possess a 5’ triphosphate.
Product details
| Catalog No | N-7001 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 21,000 Lmol-1cm-1 at 254 nm |
| Molecular Formula | C21H30N10O18P3 (free acid) |
| Molecular Weight | 803.40 g/mole (free acid) |
| Salt Form | NH4+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | m7G(5')ppp(5')G Cap Analog, N7-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine |
| Application | CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases |
| Backbone | 5'-5'-Triphosphate |
| Base Analog(s) | Guanosine |
| Nucleotide Category | Cap analogs |
| Cap Analogs | Other |
Technical documents
N-7001 Safety Data Sheet
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Intellectual property
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References
- Karikó, Katalin; Buckstein, Michael; Ni, Houping; Weissman, Drew . Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.
- Filipowicz, W. . Functions of the 5,-terminal m7G cap in eukaryotic mRNA.
- Banerjee, A. K. . 5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
- Miura, K. . The cap structure in eukaryotic messenger RNA as a mark of a strand carrying protein information.
- Zan-Kowalczewska, M.; Bretner, M.; Sierakowska, H.; Szczesna, E.; Filipowicz, W.; Shatkin, A. J. . Removal of 5'-terminal m7G from eukaryotic mRNAs by potato nucleotide pyrophosphatase and its effect on translation.
- Konarska, M. M.; Padgett, R. A.; Sharp, P. A. . Recognition of cap structure in splicing in vitro of mRNA precursors.
- Rosenberg, M.; Paterson, B. M. . Efficient cap-dependent translation of polycistronic prokaryotic mRNAs is restricted to the first gene in the operon.
- Furuichi, Y.; Miura, K. . A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus.
- Shuman, S. . Capping enzyme in eukaryotic mRNA synthesis.
- Hodel, A. E.; Gershon, P. D.; Shi, X.; Wang, S. M.; Quiocho, F. A. . Specific protein recognition of an mRNA cap through its alkylated base.
- Hodel, A. E.; Gershon, P. D.; Quiocho, F. A. . Structural basis for sequence-nonspecific recognition of 5'-capped mRNA by a cap-modifying enzyme.
- Ro-Choi, T. S. . Nuclear snRNA and nuclear function (discovery of 5' cap structures in RNA).
- Furuichi, Y.; Shatkin, A. J. . Viral and cellular mRNA capping: past and prospects.
- Matsuo, Hiroshi; Moriguchi, Tomohisa; Takagi, Toshimitsu; Kusakabe, Takahiro; Buratowski, Stephen; Sekine, Mitsuo; Kyogoku, Yoshimasa; Wagner, Gerhard . Efficient Synthesis of13C,15N-Labeled RNA Containing the Cap Structure m7GpppA
