N6-Methyladenosine-5'-Triphosphate - (N-1013)
N6-Methyl-ATP, m6ATP
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N-1013
Description
N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.). In vitro studies showed that N6-methyl ATP substituted for ATP and supported cytoskeletal filament-driven translocation of motor proteins (dynein, kinesin and myosin) (Schliwa et al. & Shimizu et al.).
N6-methyl ATP is also a substrate for RNA polymerase and can be used for preparation of modified RNA (Rohayem & Kariko et al.).
Product details
| Catalog No | N-1013 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 15,567 Lmol-1cm-1 at 265 nm |
| Molecular Formula | C11H18N5O13P3 (free acid) |
| Molecular Weight | 521.20 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | N6-Methyl-ATP, m6ATP |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Adenosine |
| Sugar Type(s) | RNA |
| Nucleotide Category | Base Modified RNA |
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References
- Missiaen, L.; Parys, J. B.; Smedt, H. D.; Sienaert, I.; Sipma, H.; Vanlingen, S.; Maes, K.; Casteels, R. . Effect of adenine nucleotides on myo-inositol-1,4,5-trisphosphate-induced calcium release.
- Karikó, Katalin; Muramatsu, Hiromi; Welsh, Frank A.; Ludwig, János; Kato, Hiroki; Akira, Shizuo; Weissman, Drew . Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.
- Karikó, Katalin; Buckstein, Michael; Ni, Houping; Weissman, Drew . Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.
- Anderson, Bart R.; Muramatsu, Hiromi; Nallagatla, Subba R.; Bevilacqua, Philip C.; Sansing, Lauren H.; Weissman, Drew; Karikó, Katalin . Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation.
- Anderson, Bart R.; Muramatsu, Hiromi; Jha, Babal K.; Silverman, Robert H.; Weissman, Drew; Karikó, Katalin . Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L.
- Motorin, Yuri; Helm, Mark . RNA nucleotide methylation.
- Alarcón, Claudio R.; Lee, Hyeseung; Goodarzi, Hani; Halberg, Nils; Tavazoie, Sohail F. . N6-methyladenosine marks primary microRNAs for processing.
- Durbin, Ann Fiegen; Wang, Chen; Marcotrigiano, Joseph; Gehrke, Lee . RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling.
- Wang, Haiyan; Qi, Chunling; He, Wenhuan; Wang, Minghui; Jiang, Wenjing; Yin, Huanshun; Ai, Shiyun . A sensitive photoelectrochemical immunoassay of N(6)-methyladenosine based on dual-signal amplification strategy: Ru doped in SiO(2) nanosphere and carboxylated g-C(3)N(4).
- Potapov, Vladimir; Fu, Xiaoqing; Dai, Nan; Corrêa, Ivan R. Jr; Tanner, Nathan A.; Ong, Jennifer L. . Base modifications affecting RNA polymerase and reverse transcriptase fidelity.
- Lee, JH;Wang, R;Xiong, F;Krakowiak, J;Liao, Z;Nguyen, PT;Moroz-Omori, EV;Shao, J;Zhu, X;Bolt, MJ;Wu, H;Singh, PK;Bi, M;Shi, CJ;Jamal, N;Li, G;Mistry, R;Jung, SY;Tsai, KL;Ferreon, JC;Stossi, F;Caflisch, A;Liu, Z;Mancini, MA;Li, W; . Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation
- Chen, YG;Chen, R;Ahmad, S;Verma, R;Kasturi, SP;Amaya, L;Broughton, JP;Kim, J;Cadena, C;Pulendran, B;Hur, S;Chang, HY; . N6-Methyladenosine Modification Controls Circular RNA Immunity
- Liu, H;Begik, O;Lucas, MC;Ramirez, JM;Mason, CE;Wiener, D;Schwartz, S;Mattick, JS;Smith, MA;Novoa, EM; . Accurate detection of m6A RNA modifications in native RNA sequences
- Zhang, X;Wang, Y;Zhou, X; . A Ligation-Based qPCR Amplification Assay for the Radiolabeling-Free Detection of ATP/NAD+ with High Selectivity and Sensitivity
- Kim, K;van Galen, P;Hovestadt, V;Rahme, G;Andreishcheva, E;Shinde, A;Gaskell, E;Jones, D;Shema, E;Bernstein, B; . Systematic detection of m6A-modified transcripts at single-molecule and single-cell resolution
- Kontur, C; . RNA Methylation and Ythdf Readers in Posttranscriptional Regulation and Development
- Valentini, P;Pierattini, B;Zacco, E;Mangoni, D;Espinoza, S;Webster, N;Andrews, B;Carninci, P;Tartaglia, G;Pandolfini, L;Gustincich, S; . Towards SINEUP-based therapeutics: design of an in vitro synthesized SINEUP RNA
- Aditham, A;Shi, H;Guo, J;Zeng, H;Zhou, Y;Wade, SD;Huang, J;Liu, J;Wang, X; . Chemically Modified mocRNAs for Highly Efficient Protein Expression in Mammalian Cells
