N6-Methyladenosine-5'-Triphosphate

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N-1013
N6-Methyl-ATP, m6ATP
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SKU Unit Size Price Qty
N-1013-1 1 µmole
$62.00
N-1013-5 5 µmole
$308.00
N-1013-10 10 µmole
$501.00
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Description

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.). In vitro studies showed that N6-methyl ATP substituted for ATP and supported cytoskeletal filament-driven translocation of motor proteins (dynein, kinesin and myosin) (Schliwa et al. & Shimizu et al.). N6-methyl ATP is also a substrate for RNA polymerase and can be used for preparation of modified RNA (Rohayem & Kariko et al.).

Product Details

Catalog No N-1013
Purity ≥90% by AX-HPLC
Extinction Coefficient 15,567 Lmol-1cm-1 at 265 nm
Molecular Formula C11H18N5O13P3 (free acid)
Molecular Weight 521.20 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) N6-Methyl-ATP, m6ATP
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Adenosine
Sugar Type(s) RNA
Nucleotide Category Base Modified RNA

Technical Documents




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References

Missiaen, L.; Parys, J. B.; Smedt, H. D.; Sienaert, I.; Sipma, H.; Vanlingen, S.; Maes, K.; Casteels, R. . Effect of adenine nucleotides on myo-inositol-1,4,5-trisphosphate-induced calcium release.

Karikó, Katalin; Muramatsu, Hiromi; Welsh, Frank A.; Ludwig, János; Kato, Hiroki; Akira, Shizuo; Weissman, Drew . Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.

Karikó, Katalin; Buckstein, Michael; Ni, Houping; Weissman, Drew . Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.

Anderson, Bart R.; Muramatsu, Hiromi; Nallagatla, Subba R.; Bevilacqua, Philip C.; Sansing, Lauren H.; Weissman, Drew; Karikó, Katalin . Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation.

Anderson, Bart R.; Muramatsu, Hiromi; Jha, Babal K.; Silverman, Robert H.; Weissman, Drew; Karikó, Katalin . Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L.

Motorin, Yuri; Helm, Mark . RNA nucleotide methylation.

Alarcón, Claudio R.; Lee, Hyeseung; Goodarzi, Hani; Halberg, Nils; Tavazoie, Sohail F. . N6-methyladenosine marks primary microRNAs for processing.

Durbin, Ann Fiegen; Wang, Chen; Marcotrigiano, Joseph; Gehrke, Lee . RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling.

Wang, Haiyan; Qi, Chunling; He, Wenhuan; Wang, Minghui; Jiang, Wenjing; Yin, Huanshun; Ai, Shiyun . A sensitive photoelectrochemical immunoassay of N(6)-methyladenosine based on dual-signal amplification strategy: Ru doped in SiO(2) nanosphere and carboxylated g-C(3)N(4).

Potapov, Vladimir; Fu, Xiaoqing; Dai, Nan; Corrêa, Ivan R. Jr; Tanner, Nathan A.; Ong, Jennifer L. . Base modifications affecting RNA polymerase and reverse transcriptase fidelity.