Use TriLink biotin monophosphates for functionalization of RNA through in vitro transcription. Use our biotin dNTPs for efficient uniform labeling in PCR.
Labeled nucleoside monophosphates enable capture and enrichment during procedures such as aptamer development by SELEX. For example, use of 5'-biotin-labeled nucleotides in in vitro transcription reactions yield transcribed aptamers containing biotin at the 5' terminus, which allows their subsequent immobilization on a avadin-coated chip.
Labeled nucleoside triphosphates can be incorporated into nucleic acids during amplification, such as PCR or in vitro transcription, to incorporate a biotin handle for use in affinity capture/purification, hybrid capture, immunoassays, tracer labeling, and nucleic acid detection. Our biotinylated nucleoside triphosphates are modified in positions that will not affect the ability of the base to pair with its partner. We also offer dye-labeled nucleoside triphosphates for incorporation into and fluorescent tracking of nucleic acids in applications such as flow cytometry and microscopy. These fluor-labeled nucleic acids provide a direct means for quantitative analysis of transfection efficiency in vitro.
To dissociate the biotinylated strand from the affinity support using mild conditions, use the biotin analog desthiobiotin. Desthobiotin lacks the sulfur ring of biotin, which reduces its binding affinity to avidin/streptavidin by several orders of magnitude and allows for competitive elution using an excess of native biotin.