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Technical Articles

The TriLink Team has developed the following library of technical articles as a resource to our customers. Is there a related topic you would like to learn more about? Let us know.

DNA Synthesis and Reagents
  A Short History of Oligonucleotide Synthesis
Troubleshooting the Synthesis of Modified Oligonucleotides
DNA Linker and Spacer Reagents and Their Utility
Phthalimidyl Protected 3' Amine Reagent Enhances Yield of Conjugated Product
Comparison of Deprotection Methods for the Phthalimidyl Amino CPGs
DADE: A Pre-Activated Carboxyl Linker, Applications and Methods
Use of Custom Synthesized Phosphoramidite Reagents
Oligonucleotide Applications
Randomer Oligonucleotides
Technical Resources
Pseudo-complementary Nucleic Acids
Understanding Oligonucleotide Synthetic Yields
Thermostability of Modified Oligonucleotide Duplexes
When is my Oligonucleotide Pure Enough?
Oligo Data and Tables
  An Introduction to Extinction Coefficients and Molecular Weights of Oligos
Nucleoside Triphosphates
  PCR Incorporation of Modified dNTPs: The Substrate Properties of Biotinylated dNTPs
Modified Nucleoside Triphosphate Applications: An Overview of the SELEX Process
Enzymatic Activity of Selected Nucleoside 5'-Triphosphates and Their Analogs
31P Chemical Shifts in NMR Spectra of Nucleotide Derivatives
Nucleoside Triphosphate Bibliography
CleanTag™ Small RNA Library Prep
  Increasing sensitivity of small RNA next generation sequencing
CleanAmp™ Applications
Improved PCR Specificity With Hot Start PCR Primers
Improved PCR flexibility with Hot Start dNTPs
Robust PCR amplification of GC-rich targets with Hot Start 7-deaza-dGTP
Reproducible Multiplex Amplification Data in 24 Minutes
mRNA & Long RNA Resources
  Anatomy of an mRNA
Transcription Reaction Basics
Aptamer Resources
  Modified Nucleoside Triphosphate Applications: An Overview of the SELEX Process
Validation of Random Library for Aptamer Selection

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