Contact Us

Ribonucleoside-5'-Triphosphate Set with 5-Methoxy-UTP - (N-1507)

NTP Set
In stock
default
N-1507
Product SKU Unit Size Price Qty
N-1507-100 100 µmole
$3,275.00
Description

Ribonucleoside triphosphates (rNTPs) are the building blocks of RNA molecules and are essential to the success of RNA drug development. TriLink’s rNTPs are manufactured under stringent quality standards in an ISO-9001 certified facility, offering exceptional quality, purity, and consistency.  They are ideally suited for RNA synthesis in research, diagnostics, therapeutics, and vaccine development. 

Substituting uridines with modified uridines, such as N1-methylpseudouridine, 5-methoxyuridine, and pseudouridine, can reduce immunogenic response and enhance translational efficiency of mRNAs. These properties can result in safer mRNA and increased protein expression. 

The rNTP set with 5-methoxy-UTP contains separate tubes of 100 mM ATP, CTP, GTP, and 5mo-UTP in H2O, pH 7.5. The rNTPs can be used with TriLink’s patented CleanCap® capping technology in in vitro transcription for robust RNA manufacturing. 

Product details
Catalog No N-1507
pH 7.5±0.5
Components Adenosine-5'-Triphosphate, Cytidine-5'-Triphosphate, Guanosine-5'-Triphosphate, 5-Methoxy-5'-Triphosphate
Concentration 100 mM
Purity ≥99% by AX-HPLC
Recommended Storage -20°C or below
Application Aptamers, In vitro Transcription, Laboratory Developed Tests (LDT) & Analyte Specific Reagents (ASR), PCR & RT-PCR
Backbone Phosphodiester
Base Analog(s) Adenosine, Cytidine, Guanosine, Uridine
Sugar Type(s) RNA
Other Name(s) NTP Set
Technical documents

Safety Data Sheet Look-up

Product Insert for mRNA Synthesis

Product Insert for saRNA Synthesis

Products FAQs

A nucleoside contains only the base and the sugar (5'-OH) while a nucleotide refers to a nucleoside with phosphorylation on the sugar. This includes the phosphate groups on the 2', 3' or 5' ends.

We provide custom chemistry services. Please inquire about the feasibility of your desired nucleotide at trilinkbiotech.com/custom-chemistry-services.

They are resuspended in water, pH 7.5±0.5.

A 1 µmole vial of 100 mM NTP contains 10 µL.

In general, we recommend equimolar amounts of nucleotides in IVT. The exact amounts depend on the IVT reagents and conditions. Please see our rNTP product inserts for IVT with CleanCap M6 and CleanCap AU analogs.

Yes, it can. Our product inserts have recommended ratios for small-scale IVT. If you would like to discuss it further, please contact us at trilinkbiotech.com/contact-us or [email protected].

When stored properly at or below -20°C, NTPs should be stable for a few years. Upon first use, prepare aliquots to minimize freeze-thaw cycles and NTP degradation. 

Although NTPs are stable at room temperature for several days, they are shipped on wet or dry ice to ensure stability in the event of a transportation delay. Even if they have arrived thawed, they should still be good to use. If you have concerns or questions however, please contact us at trilinkbiotech.com/contact-us or [email protected].

Each NTP is accompanied by a certificate of analysis with the methods of analysis, concentration, purity, identity, etc. Our GMP NTPs undergo further analyses for characterization, safety, and nuclease contamination.

We run 31P NMR, which reveals inorganic phosphate salt. Enzymologists usually have the most concern about this salt since it inhibits many polymerases.

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.