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T7 RNA Polymerase - (E-0053)

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E-0053
Product SKU Unit Size Price Qty
E-0053-01 100 µg
$265.00
E-0053-08 840 µg
$2,016.00
Description

Wild-type bacteriophage T7 RNA polymerase is a highly processive, 100 kDa, DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. It is a key enzyme used to synthesize mRNA (messenger RNA) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its high robustness and reproducibility makes it the standard industry choice for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies. 

The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. 

Product details
Catalog No E-0053
Buffer 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% glyerol, 0.1% Tween-20, pH 8.0
Reaction Sizes 25, 250
Volume 0.5 mL, 4.2 mL
Concentration 0.2 mg/mL, approximately 50 U/μL
Host E. coli
Recommended Storage -20 to -25°C
Technical documents

Safety Data Sheet Look-up

E-0053 Product Insert

Products FAQs

No, this is a standard wild type with no modifications. 

Yes

ISO 13485 compliant.

The yield is dependent on the IVT conditions (pulse vs traditional IVT) as well as inputs such as Cap, NTPs and modified NTP concentration as well as the amount of DNA template, and Polymerase.

Yes, if you don’t linearize, the transcription will continue multiple times and you will not get the expected mRNA product.

Typically, it may be due to RNase contamination, as well as salt concentration and pH.