Back to top

Hot Start PCR & RT-PCR Reagents

Polymerase Chain Reaction, PCR, revolutionized biological research in 1983. Since then, the ability and demand to amplify DNA in high throughput ways has become a basis for countless biochemical technologies. With this demand comes the responsibility to maintain accuracy and specificity in all reactions.

CleanAmp® Hot Start PCR is a TriLink patented technology developed under SBIR grants from the NIH. CleanAmp Hot Start PCR products provide a specific, sensitive, and flexible alternative to Hot Start DNA polymerases. TriLink has applied their expertise in modified nucleic acid chemistry to develop chemically modified dNTPs that enable Hot Start PCR using standard Taq DNA polymerase. Simply swap out the standard dNTPs in your PCR assay for the corresponding CleanAmp product to create a Hot Start PCR reaction. CleanAmp products are ideal for applications such as reverse transcription-PCR (RT-PCR), GC rich PCR, multiplex PCR, digital PCR, fast PCR, end-point PCR and real-time PCR (including real-time RT-PCR and fast real-time PCR). CleanAmp Hot Start PCR products offer: 

  • Compatibility with all DNA polymerases and PCR applications 
  • Unparalleled specificity and sensitivity due to significantly reduced non-specific amplification 
  • Easy assay development with minimal optimization 
  • Increased product yield 
  • High amplification efficiency with short and long amplicons (up to 23kb) 
  • Significant cost savings over other Hot Start technologies, including affordable licensing opportunities

Explore Hot Start PCR & RT-PCR Reagents

CleanAmp Master Mixes

Hot start, ready-to-use master mixes featuring CleanAmp® dNTPs.

Learn more

CleanAmp dNTPs

Turn any PCR assay into an economically-friendly Hot Start reaction by replacing standard dNTPs with CleanAmp® dNTPs.

Learn more