Search results for: 'cas9'

CRISPR-based genome editing tools show tremendous potential as therapeutic agents for the treatment of genetic diseases and viral infections, as discussed in previous  Zone   blogs  and the  June 2021 TriLink Research Spotlight  titled,  A Novel Base-Editing Strategy Promises to Treat Sickle Cell Disease . Nevertheless, safe and efficient delivery of CRISPR tools to target tissues continues to attract growing attention (see Chart). A wide variety of technical approaches are being exp...

The discovery of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has revolutionized the field of molecular biology and medicine (reviewed by Kantor et al. ) . CRISPR-mediated genome editing initially involved the generation of a Cas9-induced double-strand DNA  repaired by either non-homologous end joining (NHEJ) mechanisms, or by homology-directed repair (HDR). The discovery of the clustered regularly interspaced short palindromic repeats (CRISP...