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Search results for: 'Cre mRNA'

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    We offer a variety of gene editing tools including Cas9, Cas9 nickase and mRNAs, as well as Cre recombinases for traditional recombination. View all!
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    Introduction   Synthetic mRNA produced by in vitro transcription (IVT) is the active pharmaceutical ingredient of approved vaccines and of many drugs under development. IVT mRNA typically contains several hundred bases of non-coding untranslated regions (UTRs) that are involved in the stabilization and translation of the mRNA. Remarkably, new research has discovered that these UTRs can be virtually eliminated without compromising translation of the encoded protein of interest. ...
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    Posted in the  Zone  on  May 25, 2021 , part 1 of this series on optimizing the performance of  in vitro  translated (IVT) mRNA using N1-methylpseudouridine (N1mΨ) provided a historical introduction to the development of this chemically modified base. Part 1 focused on a 2015 study and an initial 2018  in vivo  optimization study of N1mΨ-mRNA. This Part 2 blog features a subsequent 2019 publication on  in vitro   optimization of N1mΨ-mRNA for future heart gene therapy. Befor...

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