Search results for: 'mrna builder'
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Historically, reporter genes  have been delivered as DNA plasmids for transcription into mRNA before translation into a reporter protein. In biological systems, ideal reporter proteins generate highly sensitive signals that are easily measurable. Thus, they are often photoluminescent  (e.g., GFP) or chemiluminescent  (e.g., luciferase).
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TriLink recently introduced plasmid manufacturing services  as advantageous entry points for the in vitro  transcription (IVT) template production of your mRNA, and this prompted the Zone  to provide a series of blogs exploring plasmid biology and manufacturing. This initial (Part 1) blog offers some introductory comments on the discovery of plasmids that paved the way for recombinant DNA and early IVT mRNA technologies. Part 2, which will be posted in July of 2021, transitions to d...
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This blog provides some perspective on published strategies for this type of sequence optimization, which is alternatively referred to sequence engineering. This topic has been trending upwards in recent years, as indicated by the linear forecast dashed line shown in this chart reports found by word searching the NIH PubMed database for 2013 – 2020.
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