Enhanced mRNA production by in vitro transcription and co-transcriptional capping
Increasing mRNA yields using CleanCap® M6 pulse-feed protocol
In vitro transcription (IVT) is a widely used method for producing mRNAs for research and therapeutic development. However, the mRNA cap, crucial for stability and translation, is not encoded in the template and is not incorporated during IVT by default. TriLink's CleanCap® technology offers an economical and efficient solution for co-transcriptional capping, adding the cap directly to the mRNA during the IVT process.
CleanCap® M6 analog is the latest addition to the CleanCap portfolio and enhances protein expression by more than 30% over its predecessors. This technical note reviews the IVT kinetics of CleanCap M6 and delves into its supplementary pulse-feed protocol, which increases mRNA yields and can further lower mRNA manufacturing costs.
Topics in this technical note include:
- Overview of CleanCap cap analogs
- Optimized IVT protocol using CleanCap M6
- Pulse-feed protocol to increase mRNA yields
- Assessment of IVT critical quality attributes using CleanCap M6
Read the technical note
Learn how to increase IVT yields while lowering mRNA manufacturing costs using CleanCap M6 analog.
