My Account | Shopping Cart | Contact  
Trilink Biotech Logo

Oligonucleotides | mRNA & Long RNA | Nucleotides | CleanCap® | CleanTag® | CleanAmp® | Custom Chemistry | GMP
modified oligo
Oligonucleotides

DNA Oligos
RNA Oligos
Modified Oligos
- Modified Bases
- TINA Oligos
- Light-Activated Oligos
- tRNA
Specialty Oligos
Stocked Oligos
Oligos for IVD & Molecular Dx
Manufacturing
Aptamers
Mid-Scale Synthesis
GMP Manufacturing

OligoBuilder®
Request a Quote
FAQs


 

Light-Activated Oligonucleotides For Gene Regulation, PCR, Mutagenesis & More!
Coming Soon! Please inquire for a quotation and availability of early access material.

These uniquely modified oligonucleotides are useful in a variety of applications, including:

Light-activated oligonucleotides contain photolabile nucleobase modifications, nitropiperonyloxymethyl (NPOM), to one or more of
the thymidines. The presence of the NPOM-caging group interrupts hybridization by sterically blocking Watson-Crick base pairing.
Light activation by UV irradiation specifically removes the NPOM-caging group without damaging the oligonucleotide.
The ability to regulate DNA:DNA or DNA:RNA hybridization with light provides a powerful alternative for assay activation.


npom
Figure adapted from reference 3.
The NPOM-caging group technology was invented in the Deiters Lab at North Carolina State University.

References:
1. Activation and Deactivation of DNAzyme and Antisense Function with Light for the Photochemical Regulation of Gene Expression in Mammalian Cells
Young, D. D.; Lively, M. O.; Deiters, A. J. Am. Chem. Soc. 2010, 132, 6183-6193.
2. Light Activation as a Method of Regulating and Studying Gene Expression
Deiters, A. Curr. Opin. Chem. Biol. 2009, 13, 678-686.
3. Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents
Young, D. D.; Lusic, H.; Lively, M. O.; Yoder, J. A.; Deiters, A. ChemBioChem 2008, 9, 2937-2940.
4. Light-Triggered Polymerase Chain Reaction
Young, D. D.; Edwards, W. F.; Lusic, H.; Lively, M. O.; Deiters, A. Chem. Commun. 2008, 462-464.  
5. Restriction Enzyme-Free Mutagenesis via the Light Regulation of DNA Polymerization
Young, D. D.; Lusic, H.; Lively, M. O.; Deiters, A. Nucl. Acids Res. 2009, e58.

Click here to view more publications.

  Twitter Twitter Facebook Facebook Linked In Linked In RSS RSS Google RSS  
© 2018 TriLink BioTechnologies, LLC | All Rights Reserved