Light-Activated Oligonucleotides For Gene Regulation, PCR, Mutagenesis & More!
Light-activated oligonucleotides contain photolabile nucleobase modifications, nitropiperonyloxymethyl (NPOM), to one or more of
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These uniquely modified oligonucleotides are useful in a variety of applications, including:
the thymidines. The presence of the NPOM-caging group interrupts hybridization by sterically blocking Watson-Crick base pairing.
Light activation by UV irradiation specifically removes the NPOM-caging group without damaging the oligonucleotide.
The ability to regulate DNA:DNA or DNA:RNA hybridization with light provides a powerful alternative for assay activation.
Figure adapted from reference 3.
The NPOM-caging group technology was invented in the Deiters Lab at North Carolina State University.
1. Activation and Deactivation of DNAzyme and Antisense Function with Light for the Photochemical Regulation of Gene Expression in Mammalian Cells
Young, D. D.; Lively, M. O.; Deiters, A. J. Am. Chem. Soc. 2010, 132, 6183-6193.
2. Light Activation as a Method of Regulating and Studying Gene Expression
Deiters, A. Curr. Opin. Chem. Biol. 2009, 13, 678-686.
3. Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents
Young, D. D.; Lusic, H.; Lively, M. O.; Yoder, J. A.; Deiters, A. ChemBioChem 2008, 9, 2937-2940.
4. Light-Triggered Polymerase Chain Reaction
Young, D. D.; Edwards, W. F.; Lusic, H.; Lively, M. O.; Deiters, A. Chem. Commun. 2008, 462-464.
5. Restriction Enzyme-Free Mutagenesis via the Light Regulation of DNA Polymerization
Young, D. D.; Lusic, H.; Lively, M. O.; Deiters, A. Nucl. Acids Res. 2009, e58.
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