2',3'-Dideoxyadenosine-5'-Triphosphate

Description

2',3'-Dideoxyadenosine-5'-Triphosphate (ddATP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddATP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University

Product Details

Catalog No	N-4001
Purity	≥95% by AX-HPLC
Extinction Coefficient	15,400 Lmol^-1cm^-1 at 258 nm
Molecular Formula	C₁₀H₁₆N₅O₁₁P₃ (free acid)
Molecular Weight	475.10 g/mole (free acid)
Salt Form	Li+
Concentration	100 mM
Buffer	H₂O
Recommended Storage	-20°C or below
Other Name(s)	ddATP
Application	Aptamers
Backbone	5'-Triphosphate
Base Analog(s)	Adenosine
Nucleotide Category	Sugar Modified

Technical Documents

N-4001 Safety Data Sheet

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