2',3'-Dideoxythymidine-5'-Triphosphate - (N-4004)
ddTTP
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N-4004
Description
2',3'-Dideoxythymidine-5'-Triphosphate (ddTTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."
Michal Legiewicz Research Scientist
Yale University
Product details
| Catalog No | N-4004 |
|---|---|
| Purity | ≥95% by AX-HPLC |
| Extinction Coefficient | 9,650 Lmol-1cm-1 at 267 nm |
| Molecular Formula | C10H17N2O13P3 (free acid) |
| Molecular Weight | 466.10 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | ddTTP |
| Application | Aptamers |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Thymidine |
| Nucleotide Category | Sugar Modified |
Technical documents
N-4004 Safety Data Sheet
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Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
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References
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- Vooradi, Venkataramana; Romano, Louis J. . Effect ofN-2-Acetylaminofluorene and 2-Aminofluorene Adducts on DNA Binding and Synthesis by Yeast DNA Polymerase
- Sahu, Sudheer; LaBean, Thomas H.; Reif, John H. . A DNA Nanotransport Device Powered by Polymerase 29
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- Contreras-Galindo, Rafael; Dube, Derek; Fujinaga, Koh; Kaplan, Mark H.; Markovitz, David M. . Susceptibility of Human Endogenous Retrovirus Type K to Reverse Transcriptase Inhibitors.
- Su, Kang-Yi; Lai, Hung-Ming; Goodman, Steven D.; Hu, Wei-Yao; Cheng, Wern-Cherng; Lin, Liang-In; Yang, Ya-Chien; Fang, Woei-Horng . Application of single nucleotide extension and MALDI-TOF mass spectrometry in proofreading and DNA repair assay.
