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2-Thiouridine-5'-Triphosphate - (N-1032)

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2-Thiouridine-5'-Triphosphate (2-Thio-UTP) is a substrate for several DNA polymerases and will substitute for UTP (Nakayama et al.). 2-Thio-UTP is an inhibitor of norovirus RNA dependent RNA polymerase (Belliot et al.). RNAs prepared from 2-Thio-UTP is less immunogenic as compared to unmodified RNA. 2-Thio-U modified mRNA shows no activation of RNA-dependent protein kinase (Anderson et al.), significantly reduced levels of 2'-5'-oligoadenylate synthetase activation (Anderson et al.), reduced levels of retinoic acid-inducible protein I (Hornung et al.) and suppression of activation of Toll-like receptors (Kariko et al.). However, 2-Thio-U modified mRNAs show poor translation efficiency in cell-free systems and in cultured cells (Kariko et al., Zimmer et al.). 2-Thio-UTP is a competitive inhibitor of CTP synthetase (Scheit et al.) and a potent and selective agonist of P2Y2 human receptor (El-Tayeb, et al., Jacobson et al., Ko et al., Govindan et al.).
Product details
Catalog No N-1032
Purity ≥95% by AX-HPLC
Extinction Coefficient 13,120 Lmol-1cm-1 at 274 nm
Molecular Formula C9H15N2O14P3S (free acid)
Molecular Weight 500.20 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) 2-Thio-UTP
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Uridine
Sugar Type(s) RNA
Nucleotide Category Base Modified RNA
Technical documents

N-1032 Safety Data Sheet

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Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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  1. Veres, Z.; Tsai, L.; Scholz, T. D.; Politino, M.; Balaban, R. S.; Stadtman, T. C. . Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the labile selenium donor synthesized by the selD gene product contains selenium bonded to phosphorus.
  2. Besada, Pedro; Shin, Dae Hong; Costanzi, Stefano; Ko, Hyojin; Mathé, Christophe; Gagneron, Julien; Gosselin, Gilles; Maddileti, Savitri; Harden, T. Kendall; Jacobson, Kenneth A. . Structure-Activity Relationships of Uridine 5`-Diphosphate Analogues at the Human P2Y6Receptor
  3. Karikó, Katalin; Muramatsu, Hiromi; Welsh, Frank A.; Ludwig, János; Kato, Hiroki; Akira, Shizuo; Weissman, Drew . Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.
  4. Karikó, Katalin; Buckstein, Michael; Ni, Houping; Weissman, Drew . Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.
  5. Anderson, Bart R.; Muramatsu, Hiromi; Nallagatla, Subba R.; Bevilacqua, Philip C.; Sansing, Lauren H.; Weissman, Drew; Karikó, Katalin . Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation.
  6. Anderson, Bart R.; Muramatsu, Hiromi; Jha, Babal K.; Silverman, Robert H.; Weissman, Drew; Karikó, Katalin . Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L.
  7. Hornung, Veit; Ellegast, Jana; Kim, Sarah; Brzózka, Krzysztof; Jung, Andreas; Kato, Hiroki; Poeck, Hendrik; Akira, Shizuo; Conzelmann, Karl-Klaus; Schlee, Martin; Endres, Stefan; Hartmann, Gunther . 5'-Triphosphate RNA is the ligand for RIG-I.
  8. Govindan, Sriram; Taylor, Colin W. . P2Y receptor subtypes evoke different Ca2+ signals in cultured aortic smooth muscle cells.
  9. Nakayama, C.; Saneyoshi, M. . Utilizations of various uridine 5'-triphosphate analogues by DNA-dependent RNA polymerases I and II purified from liver nuclei of the cherry salmon (Oncorhynchus masou).
  10. Zimmer, M.; Scheit, K. H. . The effect of nucleotide analogs on cell-free gene expression.
  11. Scheit, K. H.; Linke, H. J. . Substrate specificity of CTP synthetase from Escherichia coli.
  12. Ko, Hyojin; Fricks, Ingrid; Ivanov, Andrei A.; Harden, T. Kendall; Jacobson, Kenneth A. . Structure-activity relationship of uridine 5'-diphosphoglucose analogues as agonists of the human P2Y14 receptor.
  13. Ko, Hyojin; Carter, Rhonda L.; Cosyn, Liesbet; Petrelli, Riccardo; de Castro, Sonia; Besada, Pedro; Zhou, Yixing; Cappellacci, Loredana; Franchetti, Palmarisa; Grifantini, Mario; Van Calenbergh, Serge; Harden, T. Kendall; Jacobson, Kenneth A. . Synthesis and potency of novel uracil nucleotides and derivatives as P2Y2 and P2Y6 receptor agonists.
  14. Uchida, Satoshi; Itaka, Keiji; Uchida, Hirokuni; Hayakawa, Kentaro; Ogata, Toru; Ishii, Takehiko; Fukushima, Shigeto; Osada, Kensuke; Kataoka, Kazunori . In vivo messenger RNA introduction into the central nervous system using polyplex nanomicelle.
  15. Baba, Miyuki; Itaka, Keiji; Kondo, Kenji; Yamasoba, Tatsuya; Kataoka, Kazunori . Treatment of neurological disorders by introducing mRNA in vivo using polyplex nanomicelles.
  16. Uchida, Satoshi; Kataoka, Kazunori; Itaka, Keiji . Screening of mRNA Chemical Modification to Maximize Protein Expression with Reduced Immunogenicity.
  17. Muller, Margit S.; Taylor, Colin W. . ATP evokes Ca(2+) signals in cultured foetal human cortical astrocytes entirely through G protein-coupled P2Y receptors.
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