5-Aminoallyluridine-5'-Triphosphate - (N-1062)

5-AA-UTP

This product is now available upon request as a custom synthesis. It is not maintained as a stocked item.

Description

5-(3-aminoallyl)uridine 5’-triphosphate (AA-UTP) is a cost-effective alternative to direct incorporation of a labeled-UTP when preparing non-radioactive RNA probes by in vitro transcription. AA-UTP is a good substrate for T7, T3 and SP6 RNA polymerases to easily incorporate aminoallyl-uridine residues into RNA strand (Kotaskova et al.). The primary amine of aminoallyl-uridine residues can be easily coupled with NHS-ester derivatives of biotin, fluorescent dye(s) or other molecules of interest. Dye labeled aminoallyl modified RNAs are useful in microarray analysis (t Hoen et al., Scheler et. al.) and have been used for localization of RNA in cell (Wang et. al). Chen et. al. coupled 1,10-phenanthroline to an aminoallyl modified RNA for sequence specific cleavage of nucleic acids. Since AA-UTP (and AA-CTP) maintains a strict Watson-Crick base pairing recognition it is suited for the systematic evolution of ligands by exponential enrichment (SELEX) process thus allowing an introduction of primary amino-functionalities to RNA libraries (Schoetzau et al.).

Product Details

Catalog No N-1062
Purity ≥95% by AX-HPLC
Extinction Coefficient 7,100 Lmol-1cm-1 at 290 nm
Molecular Formula C12H20N3O15P3 (free acid)
Molecular Weight 539.20 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) 5-AA-UTP
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Uridine
Sugar Type(s) RNA
Nucleotide Category Base Modified RNA

Technical Documents



Certificate of Analysis


Intellectual Property

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References

Kotaskova, Jana; Tichy, Boris; Trbusek, Martin; Francova, Hana Skuhrova; Kabathova, Jitka; Malcikova, Jitka; Doubek, Michael; Brychtova, Yvona; Mayer, Jiri; Pospisilova, Sarka . High expression of lymphocyte-activation gene 3 (LAG3) in chronic lymphocytic leukemia cells is associated with unmutated immunoglobulin variable heavy chain region (IGHV) gene and reduced treatment-free survival.

't Hoen, Peter A. C.; de Kort, Floor; van Ommen, G. J. B.; den Dunnen, Johan T. . Fluorescent labelling of cRNA for microarray applications.

Scheler, Ott; Glynn, Barry; Parkel, Sven; Palta, Priit; Toome, Kadri; Kaplinski, Lauris; Remm, Maido; Maher, Majella; Kurg, Ants . Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications.

Wang, J.; Cao, L. G.; Wang, Y. L.; Pederson, T. . Localization of pre-messenger RNA at discrete nuclear sites.

Chen, C. B.; Gorin, M. B.; Sigman, D. S. . Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA.

Schoetzau, Thomas; Langner, Josmar; Moyroud, Elisabeth; Roehl, Ingo; Vonhoff, Stefan; Klussmann, Sven . Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX.

Liu, Yu; Holmstrom, Erik; Yu, Ping; Tan, Kemin; Zuo, Xiaobing; Nesbitt, David J.; Sousa, Rui; Stagno, Jason R.; Wang, Yun-Xing . Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.

Tanaka, K;Okuda, T;Kasahara, Y;Obika, S; . Base-modified aptamers obtained by cell-internalization SELEX facilitate cellular uptake of an antisense oligonucleotide


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