5-Aminoallylcytidine-5'-Triphosphate - (N-1065)
5-AA-CTP
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N-1065
Description
5-Aminoallylcytidine-5’-triphosphate (AA-CTP) can be used in the same biochemical and diagnostic applications as 5-Aminoallyluridine-5’-triphosphate (AA-UTP) (Silverman et al., Bruning et al.). AA-CTP is a good substrate for RNA polymerase and leads to a higher transcription yield of 5-aminoallyl residues than the use of AA-UTP (Baum et al.). After transcription, 5-aminoallyl-C residues can be labeled with a fluorescent dye, biotin or other functional group through amino conjugation.
AA-CTP can also be labeled or modified prior to transcription as modified-AA-CTP derivatives are typically substrates for RNA polymerase.
Product details
| Catalog No | N-1065 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 5,041 Lmol-1cm-1 at 290 nm |
| Molecular Formula | C12H21N4O14P3 (free acid) |
| Molecular Weight | 538.20 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | 5-AA-CTP |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Cytidine |
| Sugar Type(s) | RNA |
| Nucleotide Category | Base Modified RNA |
Technical documents
N-1065 Safety Data Sheet
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Certificate of analysis
CoA search tool
Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
References
- Bruning, Oskar; Rodenburg, Wendy; Radonic, Teodora; Zwinderman, Aeilko H.; de Vries, Annemieke; Breit, Timo M.; de Jong, Mark . RNA isolation for transcriptomics of human and mouse small skin biopsies.
- Hollenstein, Marcel; Hipolito, Christopher J.; Lam, Curtis H.; Perrin, David M. . A self-cleaving DNA enzyme modified with amines, guanidines and imidazoles operates independently of divalent metal cations (M2+).
- Silverman, Scott K.; Baum, Dana A. . Use of deoxyribozymes in RNA research.
- Baum, Dana A.; Silverman, Scott K. . Deoxyribozyme-catalyzed labeling of RNA.
- Bruning, Oskar; Rodenburg, Wendy; van Oostrom, Conny T.; Jonker, Martijs J.; de Jong, Mark; Dekker, Rob J.; Rauwerda, Han; Ensink, Wim A.; de Vries, Annemieke; Breit, Timo M. . A range finding protocol to support design for transcriptomics experimentation: examples of in-vitro and in-vivo murine UV exposure.
- Wu, Connie; Li, Jiahe; Wang, Wade; Hammond, Paula T. . Rationally Designed Polycationic Carriers for Potent Polymeric siRNA-Mediated Gene Silencing.
