5-Propynyl-2'-deoxyuridine-5'-Triphosphate - (N-2017)
5-Propynyl-dUTP
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N-2017
Description
The addition of a propynyl group at the C5 position of pyrimidine nucleotides is highly duplex stabilising, resulting in a higher melting temperature of the resulting DNA-DNA or DNA-RNA hybrid.
Product details
| Catalog No | N-2017 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 11,050 Lmol-1cm-1 at 291 nm |
| Molecular Formula | C12H17N2O14P3 (free acid) |
| Molecular Weight | 506.10 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | 5-Propynyl-dUTP |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Thymidine, Uridine |
| Sugar Type(s) | DNA |
| Nucleotide Category | Base Modified DNA |
Technical documents
N-2017 Safety Data Sheet
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References
- Kutyavin, Igor V. . Use of Base-Modified Duplex-Stabilizing Deoxynucleoside 5′-Triphosphates To Enhance the Hybridization Properties of Primers and Probes in Detection Polymerase Chain Reactionâ€Â
- Zhang, Xuemei; Motea, Edward; Lee, Irene; Berdis, Anthony J. . Replication of a Universal Nucleobase Provides Unique Insight into the Role of Entropy during DNA Polymerization and Pyrophosphorolysis
