OLIGObuilder Code | {rApp.} |
---|---|
Other Name(s) | rApp, 5' Adenylation |
Design Requirements | To prevent a 5′ adenylated oligo from self-ligating, a blocking group is required on the 3′-end, such as a 2′,3′-Dideoxynucleotide or 3’ (C3) Propyl Spacer.5′ adenylated oligos require an extra purification after the adenylation step and are not compatible with heat-sensitive modifications. |
Oligo Modification Type | 5' Terminal Caps (Capped Oligos) |