Product details
| OLIGObuilder Code | {rApp.} |
|---|---|
| Other Name(s) | rApp, 5' Adenylation |
| Design Requirements | To prevent a 5′ adenylated oligo from self-ligating, a blocking group is required on the 3′-end, such as a 2′,3′-Dideoxynucleotide or 3’ (C3) Propyl Spacer.5′ adenylated oligos require an extra purification after the adenylation step and are not compatible with heat-sensitive modifications. |
| Oligo Modification Type | 5' Terminal Caps (Capped Oligos) |
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References
- Lau, N. C.; Lim, L. P.; Weinstein, E. G.; Bartel, D. P. . An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.
- Zhuang, Fanglei; Fuchs, Ryan T.; Sun, Zhiyi; Zheng, Yu; Robb, G. Brett . Structural bias in T4 RNA ligase-mediated 3'-adapter ligation.
- Sriskanda, Verl; Shuman, Stewart . Role of nucleotidyltransferase motifs I, III and IV in the catalysis of phosphodiester bond formation by Chlorella virus DNA ligase.
