Antisense & RNAi
Antisense therapy involves single stranded nucleic acids that are usually highly modified. They act by one of three general mechanisms: 1) cleavage of a DNA/RNA hybrid portion by RNase H for gene silencing, 2) steric blocking of translational initiation for gene silencing, or 3) steric blocking of splice site donors or acceptors to mediate splice switching. RNAi relies on small interfering RNAs (siRNAs) that are generally 21-23 nucleotide duplex RNAs with 2 base 3’ overhangs. Upon transfection into cells, the guide strand of the siRNA duplex is incorporated into the RNA induced silencing complex (RISC). RISC then cleaves mRNAs with homology to the guide strand, silencing them.
- R-PE Antibody All-in-One Conjugation KitCat #: A-9001
- Protein-Oligo Conjugation KitCat #: S-9011
- Protein-Protein Conjugation KitCat #: S-9010
- 6 BOC HNACat #: S-3003
- S-SS-4FB Cleavable CrosslinkerCat #: S-1037
- A4FB PhosphoramiditeCat #: S-1013
- Sulfo S-HyNic Crosslinker (Water Soluble)Cat #: S-1011
- MHPH (Maleimide HyNic) CrosslinkerCat #: S-1009
- Sulfo-S-4FB Crosslinker (Water Soluble)Cat #: S-1008
- S-4FB Crosslinker (DMF Soluble)Cat #: S-1004
- S-HyNic Crosslinker (DMF Soluble)Cat #: S-1002
- SHNH Crosslinker (HyNic for Technetium Labeling)Cat #: S-1001
- APC-Antibody Conjugation KitCat #: P-9903
- R-PE Antibody Conjugation KitCat #: P-9002
- HyNic SilaneCat #: HL-1002
- Fluorescein One-Shot Antibody Labeling KitCat #: F-9001
- ChromaLink® Digoxigenin One-Shot Antibody Labeling KitCat #: B-9014
- ChromaLink® Biotin Antibody Labeling KitCat #: B-9007-105
- ChromaLink® One-Shot Antibody Biotinylation KitCat #: B-9007
- Antibody-Oligonucleotide All-in-One Conjugation KitCat #: A-9202
- HRP Antibody All-in-One Conjugation KitCat #: A-9002