Biotin-16-Aminoallyluridine-5'-Triphosphate

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N-5005
Biotin-16-AA-UTP, Biotin-16-UTP
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SKU Unit Size Price Qty
N-5005-0250 250 nmole
$175.00
N-5005-1 1 µmole
$618.00
N-5005-2 2 µmole
$872.00
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Description

Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA. According to Fenn BJ, et al., T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide. Therefore, the number of biotinylated positions per transcript can be controlled by varying the ratio of the biotinylated NTP to the standard NTP. Biotinylated RNA probes are useful as a nonradioactive label in a number of applications including chromosome mapping, in situ hybridization and microarray hybridization. Biotin-16-U-modified RNA can be detected with a variety of streptavidin-based approaches.

Product Details

Catalog No N-5005
Purity ≥90% by AX-HPLC
Extinction Coefficient 7,100 Lmol-1cm-1 at 290 nm
Molecular Formula C32H52N7O19P3S (free acid)
Molecular Weight 963.80 g/mole (free acid)
Salt Form Li+
Concentration 10 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) Biotin-16-AA-UTP, Biotin-16-UTP
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Uridine
Sugar Type(s) RNA
Nucleotide Category Biotin Labeled

Technical Documents




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References

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Langer, P. R.; Waldrop, A. A.; Ward, D. C. . Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Fenn, B. J.; Herman, T. M. . Direct quantitation of biotin-labeled nucleotide analogs in RNA transcripts.

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