Biotin-16-Aminoallyluridine-5'-Triphosphate - (N-5005)
Biotin-16-AA-UTP, Biotin-16-UTP
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N-5005
Description
Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA. According to Fenn BJ, et al., T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide. Therefore, the number of biotinylated positions per transcript can be controlled by varying the ratio of the biotinylated NTP to the standard NTP. Biotinylated RNA probes are useful as a nonradioactive label in a number of applications including chromosome mapping, in situ hybridization and microarray hybridization. Biotin-16-U-modified RNA can be detected with a variety of streptavidin-based approaches.
Product details
| Catalog No | N-5005 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 7,100 Lmol-1cm-1 at 290 nm |
| Molecular Formula | C32H52N7O19P3S (free acid) |
| Molecular Weight | 963.80 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 10 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | Biotin-16-AA-UTP, Biotin-16-UTP |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Uridine |
| Sugar Type(s) | RNA |
| Nucleotide Category | Biotin Labeled |
Technical documents
N-5005 Safety Data Sheet
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Certificate of analysis
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Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
References
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- Kim, Kye Young; Kee, Mee K.; Chong, Seon Ah; Nam, Myeong Jin . Galanin is up-regulated in colon adenocarcinoma.
- Jelinsky, S. A.; Samson, L. D. . Global response of Saccharomyces cerevisiae to an alkylating agent.
- Langer, P. R.; Waldrop, A. A.; Ward, D. C. . Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
- Fenn, B. J.; Herman, T. M. . Direct quantitation of biotin-labeled nucleotide analogs in RNA transcripts.
- Ludwig, Holger; Mages, Jörg; Staib, Caroline; Lehmann, Michael H.; Lang, Roland; Sutter, Gerd . Role of viral factor E3L in modified vaccinia virus ankara infection of human HeLa Cells: regulation of the virus life cycle and identification of differentially expressed host genes.
- Wang, Jianping; Campbell, Iain L. . Innate STAT1-dependent genomic response of neurons to the antiviral cytokine alpha interferon.
- Wegel, Eva; Vallejos, Ruben H.; Christou, Paul; Stöger, Eva; Shaw, Peter . Large-scale chromatin decondensation induced in a developmentally activated transgene locus.
- Cheng, Chi-Ping; Nagy, Peter D. . Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro.
- Mohr, E.; Prakash, N.; Vieluf, K.; Fuhrmann, C.; Buck, F.; Richter, D. . Vasopressin mRNA localization in nerve cells: characterization of cis-acting elements and trans-acting factors.
- Stupar, R. M.; Lilly, J. W.; Town, C. D.; Cheng, Z.; Kaul, S.; Buell, C. R.; Jiang, J. . Complex mtDNA constitutes an approximate 620-kb insertion on Arabidopsis thaliana chromosome 2: implication of potential sequencing errors caused by large-unit repeats.
