Twitter Facebook Linked In Zone In Google Plus 
Research Update

Detecting & Controlling Protein Expression with mRNA Devices

Synthetic biology is a growing field that introduces exogenous, synthesized biological components to influence natural biological systems. Synthetic biology has pioneered many new therapeutic strategies, including gene and protein replacement through the introduction of modified mRNA, DNA, or modified immune or stem cells into an organism.

Beyond our current capabilities, however, is the goal to program cell fate autonomously. This requires rapiddetection and response to intracellular protein levels enabling a more precise, sensitive and sophisticated system to regulate cell fate.

Recently, published in Nucleic Acids Research, a group of Japanese researchers set out to design mRNA constructs with improved sensitivity to detect endogenous proteins in human cells, allowing the information to be transmitted to downstream synthetic translational regulatory systems.

"A protein-driven mRNA device that detects a particular target protein and regulates post-transcriptional expression of exogenous genes can be used to build complex and sophisticated gene circuits, because the output protein from the device can serve as the input protein of other circuits," the researchers explain.

The existing techniques to detect specific proteins, such as western blotting, immunostaining, and chemical probes enable the analyzation of protein expression to be characterized, however, these methods often rely on...

Read More
 
New for CRISPR Cas9
Don't limit the success of your CRISPR projects, depend on Trilink quality and capability. Our new CleanCap™ Cas9 mRNA and custom high performance synthetic guidestrands (sgRNA) with sequence specific backbone and sugar modifications ensure assay performance.
Streptavidin Mag Beads
Need the highest biotin binding capacity available for critical experiments? Accelerate your isolation process and efficiency with Nanolink™ and Magnalink™ beads and agarose.
Come and See Us
7th Cambridge Symposium Nucleic Acids Chemistry & Biology
9/3-9/6 Cambridge, UK
1st Symposium on Nucleic Acid Modifications
9/4-9/6 Mainz, DE
The New Standard for
Superior mRNA Translation

mRNA capping is a critical aspect of creating viable mRNA constructs that remain biologically active and avoid self/non-self intracellular immune stimulation. TriLink scientists recognized the limitations of current capping methodologies and developed a new high efficiency, simplified co-transcriptional process.

CleanCap™ - A Novel New mRNA Capping Technology:

  • 90 99% capping efficiency

  • Natural Cap 1 mRNA to reduce cellular innate immune response.

  • Simplified transcription process

  • Lowest cost per milligram of biologically active Cap 1 mRNA

CleanCap mRNA is in stock available for common applications & experiments in standard constructs or as a custom mRNA synthesis to your specifications.

 
ARCA EGFP
CleanCap™ EGFP
CRISPR
CRISPR
CRISPR
Images courtesy of Beniag
 
Ask An Expert

Are the requirements for using CleanCap mRNA different than ARCA mRNA?

The requirements for using CleanCap mRNA are no different than using ARCA mRNA. You can use your standard electroporation or transfection conditions. There are several advantages with using a CleanCap mRNA. The translational efficiency far exceeds ...

Read More
Zone In With Zon Blog Post

Advances in Aptamer Applications - Part 2

• Top 3 Cited Aptamer Publications Over the Past Three Years

• Jerry's Picks for Top 3 Aptamer Publications So Far This Year

• TriLink Products Cited in Numerous Aptamer Publications

View Blog Post
 

 
 
© 2017 TriLink BioTechnologies | All Rights Reserved