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Research Update

Detecting & Controlling Protein Expression with mRNA Devices

Synthetic biology is a growing field that introduces exogenous, synthesized biological components to influence natural biological systems. Synthetic biology has pioneered many new therapeutic strategies, including gene and protein replacement through the introduction of modified mRNA, DNA, or modified immune or stem cells into an organism.

Beyond our current capabilities, however, is the goal to program cell fate autonomously. This requires rapiddetection and response to intracellular protein levels enabling a more precise, sensitive and sophisticated system to regulate cell fate.

Recently, published in Nucleic Acids Research, a group of Japanese researchers set out to design mRNA constructs with improved sensitivity to detect endogenous proteins in human cells, allowing the information to be transmitted to downstream synthetic translational regulatory systems.

"A protein-driven mRNA device that detects a particular target protein and regulates post-transcriptional expression of exogenous genes can be used to build complex and sophisticated gene circuits, because the output protein from the device can serve as the input protein of other circuits," the researchers explain.

The existing techniques to detect specific proteins, such as western blotting, immunostaining, and chemical probes enable the analyzation of protein expression to be characterized, however, these methods often rely on...

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The New Standard for
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mRNA capping is a critical aspect of creating viable mRNA constructs that remain biologically active and avoid self/non-self intracellular immune stimulation. TriLink scientists recognized the limitations of current capping methodologies and developed a new high efficiency, simplified co-transcriptional process.

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CleanCap™ EGFP
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Ask An Expert

Are the requirements for using CleanCap mRNA different than ARCA mRNA?

The requirements for using CleanCap mRNA are no different than using ARCA mRNA. You can use your standard electroporation or transfection conditions. There are several advantages with using a CleanCap mRNA. The translational efficiency far exceeds ...

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