Twitter Facebook Linked In Zone In Google Plus 
Research Update

X-shredding: Man-Made Male

In the inaugural edition of The CRISPR Journal, researchers from the University of Perugia published a method called X-Shredding, which identifies key loci of the X-chromosome in order to bias sex ratio.

X-shredding is a technique that utilizes the CRISPR-Cas9 system to target the X-chromosome. This leads to endonuclease-mediated X-chromosome shredding during spermatogenesis, which manipulates the sex ratio towards males.

There has been significant interest in this method because it may provide a feasible alternative to pesticides in the control of insect populations. Blood-feeding insects are responsible for more than half a million deaths each year worldwide. The Food and Agriculture Organization of the United Nations reports that herbivorous insects are responsible for the destruction of one fifth of the world’s crops annually.

"However, to build synthetic X-shredders based on CRISPR, the selection of gRNA targets in the form of high-copy sequence repeats on the X chromosome of a given species is difficult, since such repeats are not accurately resolved in genome assemblies and cannot be assigned to chromosomes with confidence," the researchers note...

Read More
 
CRISPR Cas9 Gene Editing
Don't limit the success of your CRISPR projects. Depend on Trilink quality and capability. Our new CleanCap™ Cas9 mRNA and custom high performance synthetic guidestrands (sgRNA) with sequence specific backbone and sugar modifications ensure assay performance.
Come and See Us
Oligonucleotide & Peptide Therapeutics
March 26-28 Boston, MA
Association of Biomolecular Resource Facilities (ABRF)
April 22-25 Myrtle Beach, SC
Featured Service
Check out TriLink's Discovery Drawer, a collection of rare nucleosides, nucleotides, and other small molecules.
TriLink mRNA: Setting the Standard for Quality and Performance

TriLink's portfolio of catalog and custom synthesis mRNA has set the standard for quality and performance with our proprietary CleanCap Technology which enhances and achieves superior translation. Products include gene editing, reporter gene, and expression factor constructs, and are available in standard configurations or as a custom synthesis enabling a wide array of modification options and scales. All of TriLink's mRNA products are manufactured following a certified ISO9001:2008 quality system (cGMP also avaliable) and offer:

  • Custom tailored support to meet specific application or program needs

  • A wide variety of modification, treatment and purification options

Need More Info Contact Us
 
CleanCap™ EGFP 5moU
CRISPR
14 Day Expression Analysis with Fuse-IT-mRNA CleanCap EGFP mRNA iPS derived cardiomyocytes Cor.-4U, axiogenesis, 6 minute fusion, 0.5ug of mRNA. Images courtesy of Beniag.
 
Ask An Expert

What base modifications can be incorporated into my custom mRNA or long RNA?

TriLink offers a variety of modified rNTPs suitable for in vitro transcription. We have assessed transcription efficiency based on final product formation of a 1.9 kb transcript with the following triphosphates at 100% substitution using T7 polymerase.

Read More
Zone In With The Zon Blog Post

Top 10 Genes in the Human Genome!

• The ‘Guardian of The Genome‘ p53 Tops the List of Top 10 Genes

• Cancer-Related Genes Are Prevalent Among the Top 10

• TriLink Is Proud of Its Products Mentioned in Studies of the Top 10

View Blog Post


 

 
 
© 2018 TriLink BioTechnologies | All Rights Reserved