Adenosine-5'-Triphosphate - (N-1501)
Adenosine-5′-triphosphate (ATP), cytidine-5′-triphosphate (CTP), guanosine-5′-triphosphate (GTP), and uridine-5′-triphosphate (UTP) are unmodified ribonucleoside triphosphates, serving as essential building blocks of RNA molecules. Each of them consists of an unmodified base, a ribose, and a 5′ triphosphate group. They can be used with modified nucleotides and TriLink’s patented CleanCap® capping technology in in vitro transcription for robust RNA manufacturing.
TriLink offers both research-use-only (RUO) and good manufacturing practice (GMP) rNTPs. TriLink’s GMP rNTPs are manufactured in highly controlled environments with documented procedures, traceability of materials and processes, and strict quality control measures, providing exceptional consistency, purity, and safety.
Analytical procedures performed on TriLink’s nucleotides for quality assurance include:
The rNTPs are provided 100 mM in H2O, pH 7.5.
| Catalog No | N-1501 |
|---|---|
| pH | 7.5±0.5 |
| Purity | ≥99% by AX-HPLC |
| Extinction Coefficient | 15,400 Lmol-1cm-1 at 259 nm |
| Molecular Formula | C10H16N5O13P3 (free acid) |
| Molecular Weight | 507.10 g/mole (free acid) |
| Salt Form | Na+ |
| Concentration | 100 mM |
| Recommended Storage | -20°C or below |
| Other Name(s) | ATP |
| Application | Aptamers, In vitro Transcription, Laboratory Developed Tests (LDT) & Analyte Specific Reagents (ASR), PCR & RT-PCR |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Adenosine |
| Sugar Type(s) | RNA |
| Nucleotide Category | Unmodified |
A nucleoside contains only the base and the sugar (5'-OH) while a nucleotide refers to a nucleoside with phosphorylation on the sugar. This includes the phosphate groups on the 2', 3' or 5' ends.
A common approach is to use nucleotides with a 2′-modified ribose such as 2′- fluoro or 2′-o-methyl.
We provide custom chemistry services. Please inquire about the feasibility of your desired nucleotide at trilinkbiotech.com/custom-chemistry-services.
They are resuspended in water, pH 7.5±0.5.
A 1 µmole vial of 100 mM NTP contains 10 µL.
In general, we recommend equimolar amounts of nucleotides in IVT. The exact amounts depend on the IVT reagents and conditions. Please see our rNTP product inserts for IVT with CleanCap M6 and CleanCap AU analogs.
Yes, it can. Our product inserts have recommended ratios for small-scale IVT. If you would like to discuss it further, please contact us at trilinkbiotech.com/contact-us or [email protected].
When stored properly at or below -20°C, NTPs should be stable for a few years. Upon first use, prepare aliquots to minimize freeze-thaw cycles and NTP degradation.
Although NTPs are stable at room temperature for several days, they are shipped on wet or dry ice to ensure stability in the event of a transportation delay. Even if they have arrived thawed, they should still be good to use. If you have concerns or questions however, please contact us at trilinkbiotech.com/contact-us or [email protected].
Each NTP is accompanied by a certificate of analysis with the methods of analysis, concentration, purity, identity, etc. Our GMP NTPs undergo further analyses for characterization, safety, and nuclease contamination.
We run 31P NMR, which reveals inorganic phosphate salt. Enzymologists usually have the most concern about this salt since it inhibits many polymerases.
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Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
