Thienoguanosine-5'-Triphosphate

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N-1088
Thieno-GTP, thGTP, 2-Aminothieno[3,4-d]pyrimidine-ribonucleoside-5'-Triphosphate
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SKU Unit Size Price Qty
N-1088-1 1 µmole
$91.00
N-1088-5 5 µmole
$432.00
N-1088-10 10 µmole
$785.00
N-1088-50 50 µmole
$3,260.00
N-1088-100 100 µmole
$4,180.00
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Description

GTP with a thieno[3,4-d]pyrimidine core (Thieno-GTP) is a fluorescent nucleotide that is readily accepted by T7 polymerase as a faithful GTP surrogate. This highly isomorphic nucleoside triphosphate is able to initiate transcription and incorporate during elongation. Transcripts containing a 5' pppthG are successfully dephosphorylated with alkaline phosphatase and subsequently phosphorylated with T4 polynucleotide kinase, illustrating that other terminus-modifying enzymes tolerate this modification as well.

Importantly, emissive messenger RNAs, substituted with Thieno-G are recognized by the ribosome and are capable of fluorescently reporting discrete steps in real time during translation. Additionally, Thieno-GTP can be utilized to visualize mRNA localization and assess mRNA delivery independent of translation.

Product Details

Catalog No N-1088
Purity ≥95% by AX-HPLC
Extinction Coefficient 4,500 Lmol-1cm-1 at 316 nm
Molecular Formula C11H16N3O14P3S (free acid)
Molecular Weight 539.20 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) Thieno-GTP, thGTP, 2-Aminothieno[3,4-d]pyrimidine-ribonucleoside-5'-Triphosphate
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Guanosine
Sugar Type(s) RNA
Nucleotide Category Base Modified RNA

Technical Documents




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