mRNA CDMO services
mRNA IVT synthesis for research and discovery
Our expanded custom mRNA synthesis services are designed to meet the needs of your application and development phase. We have a solution for you regardless of where you are in your mRNA journey! Our team specializes in tailoring our support to deliver on your project requirements.
Why choose TriLink for your discovery mRNA project?
Highly customizable mRNA synthesis
TriLink’s RNA design components are highly customizable to meet your application's unique needs. mRNA design and optimization in the discovery stage set the foundation for successful scale-up and transition to GMP manufacturing.
mRNA synthesis capabilities
TriLink’s proprietary mRNA synthesis capabilities include CleanScript™ in vitro transcription method optimized to decrease dsRNA, scales ranging from milligram to multi-gram, and lengths ranging from a few hundred nucleotides to over 14 kilobases.
Modifications
CleanCap® capping technology achieves highly efficient and economical co-transcriptional mRNA capping with fewer processing steps. Leverage TriLink’s comprehensive portfolio of modified nucleotides for your mRNA program.
Purification options
Purification options include silica membrane purification, Oligo dT capture, and reverse-phase high-performance liquid chromatography. Proprietary purification processes can be customized for your application.
mRNA formats to meet your project needs
Choose the custom mRNA offering below based on your application's needs. Easily obtain the format and amount of mRNA you need, from custom sets of mRNA for screening (0.1 mg target yield per construct) and hit-to-lead optimization studies (0.1–0.5 mg target yield per construct) to standard mRNA formats for preclinical studies (1 mg–20 g per construct).
- The custom sets of 20 to 96 mRNA constructs enable the testing of multiple candidate sequences with different combinations of 5’caps, modified NTPs, and poly(A) tails.
- The custom sets of 10 to 20 mRNA constructs enable you to test different sequences while holding functional variables constant. All sequences utilize the same 5’cap, modified nucleotides, purification, and formulation options.
- The standard format offers the most comprehensive selection of modifications for customization. The standard offering includes both linear mRNA and self-amplifying RNA (saRNA). Additionally, each construct comes with the option to utilize our extensive in-house analytical services.
Are you looking for modifications, lengths, and modalities beyond what is in the table? Request a consultation.
| Phase of development | Screening | Hit-to-lead or lead optimization | Lead optimization to preclinical |
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| Product format | Custom sets of 20 to 96 mRNA constructs | Custom sets of 10 to 20 mRNA constructs | Standard (mRNA and saRNA) |
| Target yield per construct* | 0.1 mg | 0.1 to 0.5 mg | 1 mg – 20 g |
| How to get a quote | Custom mRNA set intake | Custom mRNA set intake | mRNABuilder® tool |
| Modified NTPs |
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| 5’Cap options |
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| Purification options | Silica membrane | Silica membrane |
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| Formulation options | 1 mM sodium citrate or water | 1 mM sodium citrate or water | 1 mM sodium citrate, water, or other |
| Standard QC panel (included) |
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| Add-on mRNA characterization and analysis | Not available | Not available | Each construct comes with the option to utilize our extensive in-house analytical services. |
| Release documentation** | Analytical Report (Data Report) | Analytical Report (Data Report) | Analytical Report or CoA |
| Sequence length | 200bp to 4kb | 200bp to 6kb | 200bp to >14kb |
| Price | $500 to 750 per sequence | $1000 to 1500 per sequence | Request a quote |
| TAT*** | ~2-4 weeks | ~3-5 weeks | ~4-6 weeks |
| Delivery format | Cryotubes in matrix 96-tube rack | Individual cryotubes | Individual cryotubes |
* Target yield is provided as an estimate only, as the actual yield for an individual construct can be impacted by several factors, including sequence length and composition, capping type, and modifications.
**Analytical Reports (AR) are available with quicker turnaround times, released by manufacturing, and do not include a QC/QA review. Certificates of Analyses (CoAs) include QC verification and release by QA and have a longer turnaround time than an Analytical Report.
***mRNA delivery time is dependent on the DNA template turnaround time and the complexity of the construct.
Discovery mRNA videos
Overview of discovery mRNA manufacturing.
Learn how a partnership with TriLink can help you develop and optimize the right constructs for your mRNA application
Discovery manufacturing process designed to enable seamless transfer to GMP
TriLink offers robust support as your project moves from research and discovery to preclinical phases and beyond, including:
- Phase-appropriate quality—Our quality management system is designed to deliver the appropriate quality of mRNA depending on the stage of your project, from research and discovery through cGMP mRNA manufacturing for commercial mRNA drug substance
- Analytical and QC expertise—Each mRNA manufacturing facility has its own on-site QC lab. Our analytical services team offers comprehensive method development for construct-specific assays to serve your program’s unique needs.
- Process development and tech transfer—When you partner with TriLink, your mRNA program is supported by a dedicated team of process development, manufacturing, and tech transfer experts who can help you seamlessly transition from discovery to cGMP mRNA manufacturing.
Related products and services
Catalog mRNA products
Analytical services for mRNA
CleanCap® technology portfolio
Modified and unmodified nucleotides
IVT enzymes
Resources
Request a quote on your RUO mRNA project
If you are ready to submit a custom RNA synthesis request, use the mRNAbuilder™ form.
For all other mRNA program inquiries, tell us more about your specific program needs so we can tailor our initial conversation with you. We look forward to connecting with you.
