BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang.  
BspQI’s recognition and cleavage sites: 
5' GCTCTTCN↓ 3'
3' CGAGAAGNNNN↑ 5'
BspQI is thermostable and can be used in various molecular biology applications, including generation of linear DNA templates for in vitro transcription to synthesize RNA. 
The enzyme is provided 10 U/μL in storage buffer. It is also supplied with 1.25 mL of 10X RE Reaction Buffer (500 mM Tris,1000 mM NaCl, 100 mM MgCl2, 1 mg/mL rHSA, pH 7.5 at 25°C). 
Unit definition: The amount of BspQI required to digest 1 µg of lambda DNA in 1 hour at 50°C in a 50 µL reaction.
| Catalog No | E-0097 |
|---|---|
| Buffer | 10 mM Tris, 100m M KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 g/L rHSA, 50% glycerol, pH 7.0 at 25°C |
| Reaction Sizes | 500U, 2,500U |
| Volume | 50 µL, 250 µL |
| Concentration | 10 U/µL |
| Host | E. coli |
| Recommended Storage | -15 to -25°C |
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