TriLink’s CleanCap^® AG (3′ OMe) CleanScript™ IVT Kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 x 100 µL reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes, a reaction buffer, and a control DNA template.

The kit is designed for:

• Time and cost savings — Co-transcriptional capping protocol with all necessary reagents for a single-tube reaction providing high yield, fewer steps, and less handling
• High capping efficiency —Protocol with CleanCap AG (3′ OMe) cap analog to produce an optimal cap 1 with over 95% capping efficiency
• Increased mRNA yield — Buffer and kit components formulated using TriLink’s proprietary CleanScript IVT method for up to 10 mg/mL yield (or up to 1 mg mRNA from 5 µg linear plasmid) per reaction
• Ultra-low double-stranded RNA (dsRNA) levels — up to 85% dsRNA reduction using CleanScribe RNA Polymerase(in place of wild-type T7 RNA polymerase) in combination with the CleanScript method (instead of standard IVT)
• Enhanced mRNA performance — Robust protein expression and low inflammatory responses from mRNA synthesized with CleanCap AG (3′ OMe), CleanScript method, CleanScribe RNA polymerase, and optional N1-methylpseudouridine

The kit includes: 

• CleanCap^® Reagent AG (3' OMe), 10 µmol
• Adenosine-5'-Triphosphate, 100 µmol
• Cytidine-5'-Triphosphate, 100 µmol
• Guanosine-5'-Triphosphate, 100 µmol
• Uridine-5'-Triphosphate, 100 µmol
• N1-Methyl-Pseudouridine-5'-Triphosphate, 100 µmol
• AG CleanScribe™ RNA Polymerase Mix, 250 µL
• 10X AG CleanScript™ IVT Buffer, 1 mL
• FLuc Control Plasmid, 25 µg