2',3'-Dideoxyinosine-5'-Triphosphate - (N-4017)
ddITP, Didanosine triphosphate
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N-4017
Description
2',3'-Dideoxyinosine-5'-Triphosphate (ddITP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddITP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
Product details
| Catalog No | N-4017 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 12,300 Lmol-1cm-1 at 248 nm |
| Molecular Formula | C10H15N4O12P3 (free acid) |
| Molecular Weight | 476.10 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | ddITP, Didanosine triphosphate |
| Application | Aptamers |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Guanosine, Inosine |
| Nucleotide Category | Sugar Modified |
Technical documents
N-4017 Safety Data Sheet
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Intellectual property
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References
- Ordonez, Paula; Kunzelmann, Simone; Groom, Harriet C. T.; Yap, Melvyn W.; Weising, Simon; Meier, Chris; Bishop, Kate N.; Taylor, Ian A.; Stoye, Jonathan P. . SAMHD1 enhances nucleoside-analogue efficacy against HIV-1 in myeloid cells.
- Gowda, A. S. Prakasha; Krzeminski, Jacek; Amin, Shantu; Suo, Zucai; Spratt, Thomas E. . Mutagenic Replication of N(2)-Deoxyguanosine Benzo[a]pyrene Adducts by Escherichia coli DNA Polymerase I and Sulfolobus solfataricus DNA Polymerase IV.
