2',3'-Dideoxyuridine-5'-Triphosphate

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N-4003
ddUTP
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SKU Unit Size Price Qty
N-4003-1 1 µmole
$32.00
N-4003-5 5 µmole
$134.00
N-4003-10 10 µmole
$243.00
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Description

2',3'-Dideoxyuridine-5'-Triphosphate (ddUTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddUTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University

Product Details

Catalog No N-4003
Purity ≥90% by AX-HPLC
Extinction Coefficient 10,100 Lmol-1cm-1 at 262 nm
Molecular Formula C9H15N2O13P3 (free acid)
Molecular Weight 452.10 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) ddUTP
Application Aptamers
Backbone 5'-Triphosphate
Base Analog(s) Uridine
Nucleotide Category Sugar Modified

Technical Documents




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