2',3'-Dideoxyuridine-5'-Triphosphate - (N-4003)
ddUTP
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N-4003
Description
2',3'-Dideoxyuridine-5'-Triphosphate (ddUTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddUTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."
Michal Legiewicz Research Scientist
Yale University
Product details
| Catalog No | N-4003 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 10,100 Lmol-1cm-1 at 262 nm |
| Molecular Formula | C9H15N2O13P3 (free acid) |
| Molecular Weight | 452.10 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | ddUTP |
| Application | Aptamers |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Uridine |
| Nucleotide Category | Sugar Modified |
Technical documents
N-4003 Safety Data Sheet
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Certificate of analysis
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Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
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References
- Boyle, Nicholas A.; Rajwanshi, Vivek K.; Prhavc, Marija; Wang, Guangyi; Fagan, Patrick; Chen, Fu; Ewing, Gregory J.; Brooks, Jennifer L.; Hurd, Tiffany; Leeds, Janet M.; Bruice, Thomas W.; Cook, P. Dan . Synthesis of 2`,3`-Dideoxynucleoside 5`--P-Borano-,-(difluoromethylene)triphosphates and Their Inhibition of HIV-1 Reverse Transcriptase
- Smith, Thomas M.; Lim, Siew Pheng; Yue, Kimberley; Busby, Scott A.; Arora, Rishi; Seh, Cheah Chen; Wright, S. Kirk; Nutiu, Razvan; Niyomrattanakit, Pornwaratt; Wan, Kah Fei; Beer, David; Shi, Pei-Yong; Benson, Timothy E. . Identifying initiation and elongation inhibitors of dengue virus RNA polymerase in a high-throughput lead-finding campaign.
- Contreras-Galindo, Rafael; Dube, Derek; Fujinaga, Koh; Kaplan, Mark H.; Markovitz, David M. . Susceptibility of Human Endogenous Retrovirus Type K to Reverse Transcriptase Inhibitors.
- Carrell, Samuel T.; Tang, Zhenzhi; Mohr, Sabine; Lambowitz, Alan M.; Thornton, Charles A. . Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase.
