Pseudouridine-5'-Triphosphate

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N-1019
Pseudo-UTP, 5-Ribosyl Uracil
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SKU Unit Size Price Qty
N-1019-1 1 µmole
$91.00
N-1019-5 5 µmole
$425.00
N-1019-10 10 µmole
$728.00
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Description

Pseudouridine (5-ribosyluracil) was the first modified ribonucleoside discovered. It is the most abundant natural modified RNA base, and has been deemed the "fifth nucleoside" in RNA. It can be found in structural RNAs, such as transfer, ribosomal and small nuclear RNA. Pseudouridine has been found to enhance base stacking and translation. Pseudouridine-5'-triphosphate (Pseudo-UTP) is used to impart desirable mRNA characteristics such as increased nuclease stability, increased translation or altered interaction of innate immune receptors with in vitro transcribed RNA. Pseudo-UTP, along with 5-methylcytidine-5'-triphosphate (5-methyl-CTP) has shown innate immune suppression in culture and in vivo while enhancing translation in recent publications. For example, Warren et al. determined an efficient means of reprogramming multiple human cell types using modified mRNA that can express the four primary reprogramming proteins. These cells are referred to as induced pluripotency stem cells (iPSCs). Warren et al. found that enzymatically synthesized RNA substituted with Pseudo-UTP, 5-Methyl-CTP and ARCA effectively evaded the cell’s innate immune response, a crucial component in their success. Reduced toxicity due to substitution with Pseudo-UTP, 5-Methyl-CTP was critical since it allowed repeated transfection with in vitro transcribed mRNA over several weeks. View Transcription Efficiency FAQ Kariko "We value TriLink, it is an excellent company indeed. The product is very good and the costumer service is very good too. Actually a nucleic acid chemist, my friend Dr. János Ludwig recommended me your company in the spring of 2004. That time I was looking for modified-nucleoside triphosphates to generate transcripts that would help to prove or disprove the hypothesis related to lack of immunogenicity of the heavily modified tRNAs. Anyway, he gave me a long list of companies that sell or custom synthesize modified NTP, but told me that start with TriLink, they are good. And now everybody I know who is making pseudoU RNA is getting it from you." Katalin Karikó, PhD Adjunct Associate Professor of Neurosurgery University of Pennsylvania

Product Details

Catalog No N-1019
Purity ≥97% by AX-HPLC
Extinction Coefficient 7,546 Lmol-1cm-1 at 262 nm
Molecular Formula C9H15N2O15P3 (free acid)
Molecular Weight 484.10 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) Pseudo-UTP, 5-Ribosyl Uracil
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Pseudouridine
Sugar Type(s) RNA
Nucleotide Category Base Modified RNA

Technical Documents




Intellectual Property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.



References

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Karikó, Katalin; Muramatsu, Hiromi; Welsh, Frank A.; Ludwig, János; Kato, Hiroki; Akira, Shizuo; Weissman, Drew . Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.

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